Chitin synthase inhibitor 2 (compound 2b) is a highly potent inhibitor of chitin synthase, with an IC50 value of 0.09 mM and a K i value of 0.12 mM. This compound exhibits antimicrobial activities in vitro and demonstrates synergistic or additive effects when combined with fluconazole or polyoxin B [1].

Chitin synthase inhibitor 2 (compound 2b) (0, 0.032, 0.126, and 0.25 mM) inhibits chitin synthase with inhibition constant K i value of 0.12 mM and the inhibition percent (IP) of 78.3% at 0.3 mM [1]. Chitin synthase inhibitor 2 (1-4 μg/mL) has excellent antifungal activity with MIC (minimum inhibitory concentration) values of 2, 4, 1, and 2 μg/mL against C. albicans (ATCC 76615), A. fumigatus (GIMCC 3.19), C. albicans (ATCC 90023), and A. flavus (ATCC 16870), respectively [1]. Chitin synthase inhibitor 2 (128-512 g/mL) has no antibacterial activity against MRSA (N. 315), S. aureus (ATCC 25923), B. subtilis (ATCC 6633), C. coli (JM 109), P. aeruginosa (ATCC 9027), and B. proteus (ATCC 8427) [1].