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Bz-Nle-Lys-Arg-Arg-AMC是一种荧光四肽底物,用于定量检测登革热病毒(DV)NS2B-NS3蛋白酶、黄热病病毒(YFV)NS3蛋白酶和寨卡病毒NS3蛋白酶的活性。通过水解反应观测蛋白酶的裂解产物,AMC(7-氨基-4-甲基香豆素),的释放速率从而计算酶活性,激发/发射最大值分别为340-360/440-460 nm。
Bz-Nle-Lys-Arg-Arg-AMC是一种荧光四肽底物,用于定量检测登革热病毒(DV)NS2B-NS3蛋白酶、黄热病病毒(YFV)NS3蛋白酶和寨卡病毒NS3蛋白酶的活性。通过水解反应观测蛋白酶的裂解产物,AMC(7-氨基-4-甲基香豆素),的释放速率从而计算酶活性,激发/发射最大值分别为340-360/440-460 nm。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
1 mg | ¥ 560 | 35日内发货 | |
5 mg | ¥ 2,430 | 35日内发货 | |
10 mg | ¥ 4,250 | 35日内发货 | |
25 mg | ¥ 9,320 | 35日内发货 |
产品描述 | Bz-Nle-Lys-Arg-Arg-AMC is a fluorescent tetrapeptide substrate for the quantitative detection of dengue virus (DV) NS2B-NS3 protease, yellow fever virus (YFV) NS3 protease and Zika virus NS3 protease activities. The rate of release of the cleavage product of the protease, AMC (7-amino-4-methylcoumarin), was observed by hydrolysis reaction to calculate the enzyme activity, with excitation/emission maxima at 340-360/440-460 nm, respectively. |
分子量 | 832.99 |
分子式 | C41H60N12O7 |
CAS No. | 863975-32-0 |
Smiles | CC=1C=2C(=CC(NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC(=O)C3=CC=CC=C3)CCCC)=O)CCCCN)=O)CCCNC(=N)N)=O)CCCNC(=N)N)=O)=CC2)OC(=O)C1 |
存储 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
溶解度信息 | DMF: 20.00 mg/mL (24.01 mM), Sonication is recommended. DMSO:PBS (pH 7.2) (1:1): 0.5 mg/mL (0.6 mM), < 0.1 mg/mL (insoluble) Ethanol: 20.00 mg/mL (24.01 mM), Sonication is recommended. DMSO: 20.00 mg/mL (24.01 mM), Sonication is recommended. |
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