3-Acetyl-28-N–(3–guanidinobutoxy)-oleanolic acid (compound J1) serves as an effective antimicrobial agent, displaying activity against both Gram-positive bacteria and fungi. In addition to its primary use, it can also function as an adjuvant in antibiotic treatments. The mechanism of action for compound J1 involves disrupting bacterial cell membranes, integrating into DNA, and binding with DNA gyrase. Furthermore, it has been shown to reduce microbial counts and accelerate wound healing in a mouse MRSA skin infection model.