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the lymphangiogenic factors vascular endothelial growth factor C (VEGFC) and VEGFD are cleaved by thrombin and plasmin, serine proteases generated during hemostasis and wound healing. Genetic studies reveal that platelet enhancement of lymphatic growth after wounding is dependent on the release of VEGFC, but not VEGFD, a finding consistent with high expression of VEGFC in both platelets and avian thrombocytes. VEGFC Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 17.1 kDa and the accession number is Q6FH59.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
100 μg | ¥ 2,510 | 5日内发货 | |
500 μg | ¥ 10,000 | 5日内发货 | |
1 mg | ¥ 16,800 | 5日内发货 |
生物活性 | 1. Immobilized Human VEGF-C, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 17.9ng/ml determined by ELISA (QC Test).
2. Human VEGF R3, His Tag immobilized on CM5 Chip can bind Human VEGF-C, His Tag with an affinity constant of 0.29 nM as determined in SPR assay. |
产品描述 | the lymphangiogenic factors vascular endothelial growth factor C (VEGFC) and VEGFD are cleaved by thrombin and plasmin, serine proteases generated during hemostasis and wound healing. Genetic studies reveal that platelet enhancement of lymphatic growth after wounding is dependent on the release of VEGFC, but not VEGFD, a finding consistent with high expression of VEGFC in both platelets and avian thrombocytes. VEGFC Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 17.1 kDa and the accession number is Q6FH59. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-His-Avi |
蛋白编号 | Q6FH59 |
别名 | Flt4-L,血管内皮生长因子,VEGFC,VRP,VEGF-C |
蛋白构建 | Thr103-Arg227 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE |
分子量 | 17.1 kDa (predicted). Due to glycosylation, the protein migrates to 23-30 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM MES, 150 mM NaCl (pH 6.0). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in 50mM MES, 150mM NaCI (pH 6.0). The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. |
研究背景 | the lymphangiogenic factors vascular endothelial growth factor C (VEGFC) and VEGFD are cleaved by thrombin and plasmin, serine proteases generated during hemostasis and wound healing. Genetic studies reveal that platelet enhancement of lymphatic growth after wounding is dependent on the release of VEGFC, but not VEGFD, a finding consistent with high expression of VEGFC in both platelets and avian thrombocytes. |
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