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Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. VEGF121 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 17 kDa and the accession number is P15692-9.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
100 μg | ¥ 3,400 | 5日内发货 | |
500 μg | ¥ 13,600 | 5日内发货 | |
1 mg | ¥ 22,700 | 5日内发货 |
生物活性 | Immobilized Human VEGF121, His Tag at 2μg/ml (100ul/Well). Dose response curve for Biotinylated Human VEGFR1, His Tag with the EC50 of 42ng/ml determined by ELISA. |
产品描述 | Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. VEGF121 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 17 kDa and the accession number is P15692-9. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-His-Avi |
蛋白编号 | P15692-9 |
别名 | 血管内皮生长因子,VPF,VEGFMGC70609,VEGFA,VEGF,VAS,RP1-261G23.1,MVCD1,MGC70609 |
蛋白构建 | Ala27-Arg147 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE |
分子量 | 17 kDa (predicted). Due to glycosylation, the protein migrates to 18 kDa and 22-25 kDa under reduced (R) condition, 30 kDa and 32-40 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, 250 mM Arginine (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. |
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