UCHL1 Protein, Rat, Recombinant (His)

产品编号 TMPY-02331

UCHL1 Protein, Rat, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 26.2 kDa and the accession number is Q00981.

UCHL1 Protein, Rat, Recombinant (His)

产品编号 TMPY-02331

UCHL1 Protein, Rat, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 26.2 kDa and the accession number is Q00981.

规格	价格	库存	数量
50 μg	¥ 3,820	5日内发货

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产品信息

生物活性	Measured by the hydrolysis of UbiquitinAMC. The specific activity is >100 pmoles/min/μg.
产品描述	UCHL1 Protein, Rat, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 26.2 kDa and the accession number is Q00981.
种属	Rat
表达系统	E. coli
标签	N-His
蛋白编号	Q00981
别名	ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
蛋白构建	A DNA sequence encoding the rat UCHL1 (Q00981) (Glu 2-Ala 223) was fused with a polyhistidine tag at the N-terminus. Predicted N terminal: Met
蛋白纯度	> 97 % as determined by SDS-PAGE
分子量	26.2 kDa (predicted); 28 kDa (reducing conditions)
内毒素	Please contact us for more information.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Ubiquitin carboxyl-terminal hydrolase isozyme L1, also known as UCH-L1, Ubiquitin thioesterase L1, PGP9.5 and UCHL1, is a deubiqutinating enzyme with important functions in recycling of ubiquitin. Regulated proteolysis by the ubiquitin pathway has been implicated in control of the cell cycle, transcriptional activation, cell fate and growth, and synaptogenesis. The ubiquitin-proteasome system is involved in synaptic plasticity and is proposed to be part of a molecular switch that converts short-term synaptic potentiation to long-term changes in synaptic strength. UCHL1 is found in neuronal cell bodies and processes throughout the neocortex (at protein level). It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. UCHL1 is weakly expressed in ovary. UCHL1 is a ubiquitin-protein hydrolase. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. UCHL1 dysfunction has been associated with neurodegeneration in Parkinson's, Alzheimer's, and Huntington's disease patients. Reduced UCHL1 function may jeopardize the survival of CNS neurons.