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Liver diseases such as liver cirrhosis, primary and metastatic liver cancers are still a major medical challenge. Syndecan-1 is one of the most important proteoglycans in the liver. Syndecan-1 is normally expressed on the surfaces of hepatocytes and cholangiocytes. Due to liver diseases the amount of syndecan-1 increases in the liver. Syndecan-1 Protein, Human, Recombinant (aa 23-254, hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 50.6 kDa and the accession number is AAH08765.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
100 μg | ¥ 2,660 | 5日内发货 | |
500 μg | ¥ 10,600 | 5日内发货 | |
1 mg | ¥ 17,800 | 5日内发货 |
生物活性 | Immobilized Human Syndecan-1, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-Syndecan-1 Antibody, hFc Tag with the EC50 of 19.2ng/ml determined by ELISA. |
产品描述 | Liver diseases such as liver cirrhosis, primary and metastatic liver cancers are still a major medical challenge. Syndecan-1 is one of the most important proteoglycans in the liver. Syndecan-1 is normally expressed on the surfaces of hepatocytes and cholangiocytes. Due to liver diseases the amount of syndecan-1 increases in the liver. Syndecan-1 Protein, Human, Recombinant (aa 23-254, hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 50.6 kDa and the accession number is AAH08765. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-hFc |
蛋白编号 | AAH08765 |
别名 | Syndecan-1,SYND1,SDC1,SDC,CD138 |
蛋白构建 | Gln23-Gly254 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE |
分子量 | 50.6 kDa (predicted). Due to glycosylation, the protein migrates to 70-100 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Liver diseases such as liver cirrhosis, primary and metastatic liver cancers are still a major medical challenge. Syndecan-1 is one of the most important proteoglycans in the liver. Syndecan-1 is normally expressed on the surfaces of hepatocytes and cholangiocytes. Due to liver diseases the amount of syndecan-1 increases in the liver. |
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