Osteopontin Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 33.16 kDa and the accession number is A0A2K5WL90.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 3,100 | 5日内发货 | ||
500 μg | ¥ 12,400 | 5日内发货 | ||
1 mg | ¥ 20,700 | 5日内发货 |
产品描述 | Osteopontin Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 33.16 kDa and the accession number is A0A2K5WL90. |
种属 | Cynomolgus |
表达系统 | HEK293 Cells |
标签 | C-His |
蛋白编号 | A0A2K5WL90 |
别名 | BSP-1, BNSP, SPP1, Nephropontin, OPN, Bone sialoprotein 1, ETA-1, Osteopontin, Uropontin, BSPI, SPP-1 |
蛋白构建 | Leu17-Asn300 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 33.16 kDa (predicted). Due to glycosylation, the protein migrates to 45-55 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Ovarian cancer is one of the most lethal malignant tumors in women. Secreted phosphoprotein 1 (SPP1) plays an important role in some cancer types. The expression of SPP1 was higher in epithelial ovarian cancer tissues than in normal ovarian tissues. Silencing SPP1 decreased the cell proliferation, migration, and invasion. Ectopic expression of SPP1 increased the cell proliferation, migration, and invasion. Silencing SPP1 prevented ovarian cancer growth in mice. Silencing SPP1 inhibited Integrin β1/FAK/AKT pathway. |
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Osteopontin Protein, Cynomolgus, Recombinant (His) BSP-1 BSP 1 ETA1 BNSP SPP1 ETA 1 SPP 1 BSP1 Nephropontin OPN Bone sialoprotein 1 ETA-1 Osteopontin Uropontin BSPI SPP-1 recombinant recombinant-proteins proteins protein