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SHH Protein, Mouse, Recombinant (C25II)

产品编号 TMPJ-01171

SHH Protein, Mouse, Recombinant (C25II) is expressed in E. coli expression system. The predicted molecular weight is 18-20 KDa and the accession number is Q62226.

SHH Protein, Mouse, Recombinant (C25II)

SHH Protein, Mouse, Recombinant (C25II)

产品编号 TMPJ-01171
SHH Protein, Mouse, Recombinant (C25II) is expressed in E. coli expression system. The predicted molecular weight is 18-20 KDa and the accession number is Q62226.
规格价格库存数量
10 μg
¥ 820
5日内发货
50 μg
¥ 2,480
5日内发货
500 μg
¥ 12,100
5日内发货
1 mg
¥ 17,400
5日内发货
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产品信息

生物活性
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
SHH Protein, Mouse, Recombinant (C25II) is expressed in E. coli expression system. The predicted molecular weight is 18-20 KDa and the accession number is Q62226.
种属
Mouse
表达系统
E. coli
标签Tag Free
蛋白编号Q62226
别名
Sonic Hedgehog Protein,SHH,HHG-1
氨基酸序列
Cys25-Gly198(Cys25Ile-Ile)
蛋白构建
Cys25-Gly198(Cys25Ile-Ile)
蛋白纯度
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
SHH Protein, Mouse, Recombinant (C25II)
分子量18-20 KDa (reducing condition)
内毒素< 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PB, 150 mM NaCl, 1 mM DTT, pH 7.4.
复溶方法
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景
Mouse Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called Hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases.Mouse Shh is synthesized as a 437 aa precursor that contains a 24 aa signal sequence and a 413 aa mature region. The mature region is autocatalytically processed into a nonglycosylated, 20 kDa, 174 aa N­terminal fragment (Shh­N), and a catalytic­processing,glycosylated, 34 kDa, 239 aa C­terminal fragment. The 20 kDa Shh­N fragment is the core of the active hedgehog molecule. Mouse Shh­N is 99%, 98%, and 100% aa identical to human, rat and gerbil Shh­N, respectively.

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