SHH Protein, Human, Recombinant (C24II)

SHH Protein, Human, Recombinant (C24II) is expressed in E. coli expression system. The predicted molecular weight is 20 KDa and the accession number is Q15465.

规格	价格/CNY		数量
10 μg	￥ 910	5日内发货
50 μg	￥ 2,760	5日内发货
500 μg	￥ 12,100	5日内发货
1 mg	￥ 17,400	5日内发货

规格

价格/CNY

货期

数量

10 μg

￥ 910

5日内发货

50 μg

￥ 2,760

5日内发货

500 μg

￥ 12,100

5日内发货

1 mg

￥ 17,400

5日内发货

产品描述	SHH Protein, Human, Recombinant (C24II) is expressed in E. coli expression system. The predicted molecular weight is 20 KDa and the accession number is Q15465.

种属	Human
表达系统	E. coli
标签	Tag Free
蛋白编号	Q15465
别名	HHG-1, Sonic Hedgehog Protein, SHH
氨基酸序列	Cys24-Gly197
蛋白构建	Cys24-Gly197 (C24II)
蛋白纯度	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量	20 KDa (reducing condition)

内毒素	< 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景	Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called Hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases. Human SHH is expressed as a 45kDa precursor, and undergoes a series of processing during secretion. After the removal of the signal peptide, a protease within the C-terminal domain catalyzes the cleavage of SHH into a 20 kDa N-terminal signaling domain (SHH-N) and a 25 kDa C-terminal domain (SHH-C). SHH-N has the “all signaling” capability. SHH-N binds to the 12 pass transmembrane protein Patched (Ptc) on cell surface, which releases the repression of the activity of Smoothened (Smo), a G-protein coupled receptor, by Ptc.

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Mass (in vial)

Desired Reconstitution Concentration

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SHH Protein, Human, Recombinant (C24II)

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