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R-Spondin 1/RSPO1 Protein, Human, Recombinant
产品编号 TMPY-03626
R-Spondin 1/RSPO1 Protein, Human, Recombinant is expressed in CHO mammalian cells. The predicted molecular weight is 26.8 kDa and the accession number is Q2MKA7-1.
R-Spondin 1/RSPO1 Protein, Human, Recombinant
产品编号 TMPY-03626
R-Spondin 1/RSPO1 Protein, Human, Recombinant is expressed in CHO mammalian cells. The predicted molecular weight is 26.8 kDa and the accession number is Q2MKA7-1.
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 20 μg | ¥ 1,750 | 现货 | |
| 100 μg | ¥ 4,890 | 5日内发货 | |
| 200 μg | ¥ 9,270 | 5日内发货 | |
| 500 μg | ¥ 17,800 | 5日内发货 |
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产品信息
| 生物活性 | 1. Measured by its ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 10-80 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt3a. 2. Human intestinal cancer organoids were cultured with RSPO1, EGF, NOG. (Routinely tested). 3. Human colorectum organoids were cultured with RSPO1, EGF, NOG. (Routinely tested). 4. Human lung cancer organoids were cultured with FGF2, FGF4, FGF7, EGF, FGF10, NOG, RSPO1. (Routinely tested). 5. Human lung organoids were cultured with FGF2, FGF4, FGF7,EGF, FGF10, NOG, RSPO1. (Routinely tested). 6. Human cholangiocarcinomas organoids were cultured with FGF2, HGF, FGF7, EGF, FGF10, NOG, RSPO1. (Routinely tested). 7. Human liver cancer organoids were cultured with FGF2, HGF, FGF7, EGF, FGF10, TGFB1, NOG,RSPO1. (Routinely tested). 8. Human kidney cancer organoids were cultured with FGF2, FGF7, EGF, FGF10, NOG, RSPO1. (Routinely tested). 9. Human kidney organoids were cultured with FGF7, EGF, FGF10,NOG, RSPO1, HGF, FGF4. (Routinely tested). 10. Human gastric cancer organoids were cultured with EGF, FGF10, NOG, RSPO1. (Routinely tested). 11. Human stomach organoids organoids were cultured with EGF, FGF10, NOG, RSPO1. (Routinely tested). 12. Human breast cancer organoids were cultured with FGF7, RSPO1, IGF1, EGF, NRG1 Beta 1, NOG. (Routinely tested). 13. Human ovarian organoids were cultured with IGF1, NRG1 Beta 1,RSPO1, EGF, NOG. (Routinely tested).  |
| 产品描述 | R-Spondin 1/RSPO1 Protein, Human, Recombinant is expressed in CHO mammalian cells. The predicted molecular weight is 26.8 kDa and the accession number is Q2MKA7-1. |
| 种属 | Human |
| 表达系统 | CHO Cells |
| 标签 | Tag Free |
| 蛋白编号 | Q2MKA7-1 |
| 别名 | R-spondin 1,RSPO,CRISTIN3 |
| 蛋白构建 | A DNA sequence encoding the human RSPO1 (NP_001033722.1) (Met1-Ala263) was expressed and purified. Predicted N terminal: Ser 21 |
| 蛋白纯度 | ≥ 95 % as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC   |
| 分子量 | 26.8 kDa (predicted); 40 and 31 kDa (reducing condition, due to glycosylation) |
| 内毒素 | < 10 EU/mg of the protein. |
| 缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, PH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| 复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. |
| 研究背景 | RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6. |
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