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RPE65 Protein, Human, Recombinant (His & Myc)

RPE65 Protein, Human, Recombinant (His & Myc)

产品编号 TMPH-02033
别名: Retinol isomerase, Retinoid isomerohydrolase, All-trans-retinyl-palmitate hydrolase, Meso-zeaxanthin isomerase, RPE65, Retinal pigment epithelium-specific 65 kDa protein, Lutein isomerase

RPE65 Protein, Human, Recombinant (His & Myc) is expressed in Baculovirus.

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RPE65 Protein, Human, Recombinant (His & Myc)
规格 价格/CNY 货期 数量
20 μg ¥ 3,120 20日内发货
100 μg ¥ 8,760 20日内发货
1 mg ¥ 19,400 20日内发货
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产品目录号及名称: RPE65 Protein, Human, Recombinant (His & Myc) (TMPH-02033)
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生物活性
技术参数
产品性质
产品描述 RPE65 Protein, Human, Recombinant (His & Myc) is expressed in Baculovirus.
种属 Human
表达系统 Baculovirus Insect Cells
标签 N-10xHis, C-Myc
蛋白编号 Q16518
别名 Retinol isomerase, Retinoid isomerohydrolase, All-trans-retinyl-palmitate hydrolase, Meso-zeaxanthin isomerase, RPE65, Retinal pigment epithelium-specific 65 kDa protein, Lutein isomerase
氨基酸序列 SIQVEHPAGGYKKLFETVEELSSPLTAHVTGRIPLWLTGSLLRCGPGLFEVGSEPFYHLFDGQALLHKFDFKEGHVTYHRRFIRTDAYVRAMTEKRIVITEFGTCAFPDPCKNIFSRFFSYFRGVEVTDNALVNVYPVGEDYYACTETNFITKINPETLETIKQVDLCNYVSVNGATAHPHIENDGTVYNIGNCFGKNFSIAYNIVKIPPLQADKEDPISKSEIVVQFPCSDRFKPSYVHSFGLTPNYIVFVETPVKINLFKFLSSWSLWGANYMDCFESNETMGVWLHIADKKRKKYLNNKYRTSPFNLFHHINTYEDNGFLIVDLCCWKGFEFVYNYLYLANLRENWEEVKKNARKAPQPEVRRYVLPLNIDKADTGKNLVTLPNTTATAILCSDETIWLEPEVLFSGPRQAFEFPQINYQKYCGKPYTYAYGLGLNHFVPDRLCKLNVKTKETWVWQEPDSYPSEPIFVSHPDALEEDDGVVLSVVVSPGAGQKPAYLLILNAKDLSEVARAEVEINIPVTFHGLFKKS
蛋白构建 2-533 aa
蛋白纯度 > 85% as determined by SDS-PAGE.
分子量 64.7 kDa (predicted)
缓冲液 If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
复溶方法 Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

运输方式

In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.

研究背景 Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore. Essential for the production of 11-cis retinal for both rod and cone photoreceptors. Also capable of catalyzing the isomerization of lutein to meso-zeaxanthin an eye-specific carotenoid. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT.

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Keywords

RPE65 Protein, Human, Recombinant (His & Myc) Retinol isomerase Retinoid isomerohydrolase All-trans-retinyl-palmitate hydrolase Meso-zeaxanthin isomerase RPE65 Retinal pigment epithelium-specific 65 kDa protein Lutein isomerase recombinant recombinant-proteins proteins protein

 

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