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PKM2 Protein, Human, Recombinant (His)

产品编号 TMPY-04465

别名: pyruvate kinase, muscle, OIP3, CTHBP, PK3, THBP1, HEL-S-30, TCB, PKM2

PKM2 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 59 kDa and the accession number is P14618-1.

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PKM2 Protein, Human, Recombinant (His)

规格	价格/CNY		货期	数量
50 μg	￥ 2,530		5日内发货

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参考文献

生物活性	Kinase activity untested
产品描述	PKM2 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 59 kDa and the accession number is P14618-1.

种属	Human
表达系统	E. coli
标签	N-His
蛋白编号	P14618-1
别名	pyruvate kinase, muscle, OIP3, CTHBP, PK3, THBP1, HEL-S-30, TCB, PKM2
蛋白构建	The Human PKM2 isoform M2 (P14618-1) (Ser 2-Pro 531) was expressed, with a polyhistide tag at the N-terminus.
蛋白纯度	> 90 % as determined by SDS-PAGE
分子量	59 kDa (predicted)

内毒素	Please contact us for more information.
缓冲液	Supplied as sterile PBS, pH 7.0, 10% glycerol.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
研究背景	Pyruvate kinase isozymes M2 also known as pyruvate kinase muscle isozyme 2 (PKM2), pyruvate kinase type K, cytosolic thyroid hormone-binding protein (CTHBP), thyroid hormone-binding protein 1 (THBP1), or opa-interacting protein 3 (OIP3), is an isoenzyme of the glycolytic enzyme pyruvate kinase. Pyruvate kinase isozymes M2 / PKM2 is a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. PKM2 has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. PKM2 has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. PKM2 functions as a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. PKM2 may stimulate POU5F1-mediated transcriptional activation. This protein plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms of PKM2 contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

参考文献

1. Bluemlein K, et al. (2011) No evidence for a shift in pyruvate kinase PKM1 to PKM2 expression during tumorigenesis. Oncotarget. 2 (5): 393-400. 2. Gupta V, et al. (2010) Dominant Negative Mutations Affect Oligomerization of Human Pyruvate Kinase M2 Isozyme and Promote Cellular Growth and Polyploidy. J Biol Chem. 285 (22): 16864-73. 3. Eigenbrodt E, et al. (1992) Double role for pyruvate kinase type M2 in the expansion of phosphometabolite pools found in tumor cells. Crit Rev Oncog. 3 (1): 91-115.

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Keywords

PKM2 Protein, Human, Recombinant (His) pyruvate kinase, muscle THBP-1 PK-3 PK 3 OIP3 OIP 3 CTHBP PK3 PKM-2 THBP1 HEL-S-30 THBP 1 OIP-3 TCB PKM2 PKM 2 recombinant recombinant-proteins proteins protein

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