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PARP2 Protein, Human, Recombinant (His)

产品编号 TMPH-01880

PARP2 Protein, Human, Recombinant (His) is expressed in E. coli expression system with C-10xHis tag. The predicted molecular weight is 41.3 kDa and the accession number is Q9UGN5.

PARP2 Protein, Human, Recombinant (His)

PARP2 Protein, Human, Recombinant (His)

产品编号 TMPH-01880
PARP2 Protein, Human, Recombinant (His) is expressed in E. coli expression system with C-10xHis tag. The predicted molecular weight is 41.3 kDa and the accession number is Q9UGN5.
规格价格库存数量
20 μg
¥ 1,800
20日内发货
100 μg
¥ 3,760
20日内发货
1 mg
¥ 16,000
20日内发货
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产品信息

生物活性
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
PARP2 Protein, Human, Recombinant (His) is expressed in E. coli expression system with C-10xHis tag. The predicted molecular weight is 41.3 kDa and the accession number is Q9UGN5.
种属
Human
表达系统
E. coli
标签C-10xHis
蛋白编号Q9UGN5
别名
Protein poly-ADP-ribosyltransferase PARP2,Poly[ADP-ribose] synthase 2,Poly [ADP-ribose] polymerase 2,PARP2,NAD(+) ADP-ribosyltransferase 2,DNA ADP-ribosyltransferase PARP2,ADP-ribosyltransferase diphtheria toxin-like 2
氨基酸序列
QLDLRVQELIKLICNVQAMEEMMMEMKYNTKKAPLGKLTVAQIKAGYQSLKKIEDCIRAGQHGRALMEACNEFYTRIPHDFGLRTPPLIRTQKELSEKIQLLEALGDIEIAIKLVKTELQSPEHPLDQHYRNLHCALRPLDHESYEFKVISQYLQSTHAPTHSDYTMTLLDLFEVEKDGEKEAFREDLHNRMLLWHGSRMSNWVGILSHGLRIAPPEAPITGYMFGKGIYFADMSSKSANYCFASRLKNTGLLLLSEVALGQCNELLEANPKAEGLLQGKHSTKGLGKMAPSSAHFVTLNGSTVPLGPASDTGILNPDGYTLNYNEYIVYNPNQVRMRYLLKVQFNFLQLW
蛋白构建
233-583 aa
蛋白纯度
> 85% as determined by SDS-PAGE.
分子量41.3 kDa (predicted)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
复溶方法
Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景
Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mediates glutamate, aspartate or serine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Mediates glutamate and aspartate ADP-ribosylation of target proteins in absence of HPF1. Following interaction with HPF1, catalyzes serine ADP-ribosylation of target proteins; HPF1 conferring serine specificity by completing the PARP2 active site. PARP2 initiates the repair of double-strand DNA breaks: recognizes and binds DNA breaks within chromatin and recruits HPF1, licensing serine ADP-ribosylation of target proteins, such as histones, thereby promoting decompaction of chromatin and the recruitment of repair factors leading to the reparation of DNA strand breaks. In addition to proteins, also able to ADP-ribosylate DNA: preferentially acts on 5'-terminal phosphates at DNA strand breaks termini in nicked duplex.

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