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KLRG1 Protein, Human, Recombinant (hFc)

产品编号 TMPK-01140

KLRG1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with N-hFc tag. The predicted molecular weight is 42.8 kDa and the accession number is Q96E93-1.

KLRG1 Protein, Human, Recombinant (hFc)

KLRG1 Protein, Human, Recombinant (hFc)

产品编号 TMPK-01140
KLRG1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with N-hFc tag. The predicted molecular weight is 42.8 kDa and the accession number is Q96E93-1.
规格价格库存数量
100 μg
¥ 2,960
5日内发货
500 μg
¥ 11,800
5日内发货
1 mg
¥ 19,700
5日内发货
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产品信息

生物活性
Immobilized Human KLRG1, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-KLRG1 Antibody, hFc Tag with the EC50 of 20.8ng/ml determined by ELISA.
产品描述
KLRG1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with N-hFc tag. The predicted molecular weight is 42.8 kDa and the accession number is Q96E93-1.
种属
Human
表达系统
HEK293 Cells
标签N-hFc
蛋白编号Q96E93-1
别名
MGC13600,MAFAL,MAFA,KLRG1,CLEC15AMAFA-LIKE,CLEC15A,2F1
蛋白构建
Leu60-Phe195
蛋白纯度
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量42.8 kDa (predicted). Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
内毒素< 1 EU/μg by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
Immune homeostasis requires the tight, tissue-specific control of the different CD4 Foxp3 regulatory T (Treg) cell populations. The cadherin-binding inhibitory receptor killer cell lectin-like receptor G1 (KLRG1) is expressed by a subpopulation of Treg cells with GATA3 effector phenotype.Lack of KLRG1 on Treg cells conferred on them a competitive advantage in the gut, but not in lymphoid organs. Hence, although absence of KLRG1 is not enough to increase intestinal Treg cells in KLRG1 knockout mice, KLRG1 ligation reduces T-cell receptor signals and the competitive fitness of individual Treg cells in the intestine.

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