购物车
- 全部删除
- 您的购物车当前为空
KLRG1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with N-hFc tag. The predicted molecular weight is 42.8 kDa and the accession number is Q96E93-1.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
100 μg | ¥ 2,960 | 5日内发货 | |
500 μg | ¥ 11,800 | 5日内发货 | |
1 mg | ¥ 19,700 | 5日内发货 |
生物活性 | Immobilized Human KLRG1, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-KLRG1 Antibody, hFc Tag with the EC50 of 20.8ng/ml determined by ELISA. |
产品描述 | KLRG1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with N-hFc tag. The predicted molecular weight is 42.8 kDa and the accession number is Q96E93-1. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | N-hFc |
蛋白编号 | Q96E93-1 |
别名 | MGC13600,MAFAL,MAFA,KLRG1,CLEC15AMAFA-LIKE,CLEC15A,2F1 |
蛋白构建 | Leu60-Phe195 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 42.8 kDa (predicted). Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Immune homeostasis requires the tight, tissue-specific control of the different CD4 Foxp3 regulatory T (Treg) cell populations. The cadherin-binding inhibitory receptor killer cell lectin-like receptor G1 (KLRG1) is expressed by a subpopulation of Treg cells with GATA3 effector phenotype.Lack of KLRG1 on Treg cells conferred on them a competitive advantage in the gut, but not in lymphoid organs. Hence, although absence of KLRG1 is not enough to increase intestinal Treg cells in KLRG1 knockout mice, KLRG1 ligation reduces T-cell receptor signals and the competitive fitness of individual Treg cells in the intestine. |