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IRE1 Protein, Human, Recombinant (aa 465-977)

产品编号 TMPY-04753

IRE1 Protein, Human, Recombinant (aa 465-977) is expressed in Baculovirus insect cells. The predicted molecular weight is 58.3 kDa and the accession number is O75460-1.

IRE1 Protein, Human, Recombinant (aa 465-977)

IRE1 Protein, Human, Recombinant (aa 465-977)

产品编号 TMPY-04753
IRE1 Protein, Human, Recombinant (aa 465-977) is expressed in Baculovirus insect cells. The predicted molecular weight is 58.3 kDa and the accession number is O75460-1.
规格价格库存数量
50 μg
¥ 3,170
现货
100 μg
¥ 5,420
5日内发货
200 μg
¥ 9,260
5日内发货
500 μg
¥ 18,720
5日内发货
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产品信息

生物活性
1. Kinase activity untested 2. Measured by its nuclease activity to cleave Xbp1 single stem-loop mini-substrate.
产品描述
IRE1 Protein, Human, Recombinant (aa 465-977) is expressed in Baculovirus insect cells. The predicted molecular weight is 58.3 kDa and the accession number is O75460-1.
种属
Human
表达系统
Baculovirus Insect Cells
标签Tag Free
蛋白编号O75460-1
别名
IRE1P,IRE1a,IRE1,hIRE1p,endoplasmic reticulum to nucleus signaling 1
蛋白构建
A DNA sequence encoding the human ERN1 (O75460-1) (Pro 465-Leu 977) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus. Predicted N terminal: Gly
蛋白纯度
> 80 % as determined by SDS-PAGE
IRE1 Protein, Human, Recombinant (aa 465-977)
分子量58.3 kDa (predicted); 65 kDa (reducing condition, due to glycosylation)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Supplied as sterile 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
研究背景
Endoplasmic reticulum stress and hypoxia are necessary components of malignant tumors growth and suppression of ERN1 (from endoplasmic reticulum to nuclei-1) signalling pathway, which is linked to the apoptosis and cell death processes, significantly decreases proliferative processes. An enhanced expression of TP53 gene in ERN1 knockdown glioma cells correlates with the decreased level of ubiquitin ligase MDM2 and increased expression level of USP7 which deubiquitinates TP53 and MDM2 and induces TP53-dependent cell growth repression and apoptosis. Thus, the expression of genes encoding TP53 and related to TP53 factors depends upon the endoplasmic reticulum stress signaling as well as on hypoxia, and correlates with suppression of glioma growth under ERN1 knockdown. The dependence of insulin-like growth binding proteins as well as IGF2BP3 and HTRA1 gene expressions in U87 glioma cells on ERN1 signaling enzyme function and hypoxia, indicating its participation in the regulation of metabolic and proliferative processes via IGF/INS receptors, because endoplasmic reticulum stress is an important component of tumor growth and metabolic diseases.

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