Influenza A H3N2 (A/Darwin/9/2021)/(A/Darwin/6/2021) Nucleoprotein/NP Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 57.33 kDa.

H3N2

Baculovirus Insect Cells

NP Protein

A DNA sequence encoding the Influenza A H3N2 (A/Darwin/9/2021)/(A/Darwin/6/2021) Nucleoprotein (EPI1884976) (Met1-Asn498) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Met1

> 90 % as determined by SDS-PAGE.

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Influenza viral nucleoprotein (NP) plays a critical role in virus replication and host adaptation. Nucleoprotein (NP) is an essential multifunctional protein that encapsidates the viral genome and functions as an adapter between the virus and the host cell machinery. NPs contain two nuclear localization signals (NLSs): a well-studied monopartite NLS1 and a less-characterized NLS2, thought to be bipartite. The nucleocapsid is a complex of the viral nucleoprotein, RNA, and several other viral proteins. The nucleoprotein forms large, RNA-bound, helical filaments and acts as a scaffold for additional viral proteins.