购物车
- 全部删除
- 您的购物车当前为空
IL-1R1 Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 37.4 kDa and the accession number is G8F337.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
100 μg | ¥ 3,820 | 5日内发货 | |
1 mg | ¥ 24,900 | 5日内发货 |
生物活性 | Measured by its binding ability in a functional ELISA. Immobilized Human IL1RA at 2 μg/ml (100 μl/well) can bind Cynomolgus IL1R1 His, the EC50 of Cynomolgus IL1R1 His is 2.5-15 ng/mL. |
产品描述 | IL-1R1 Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 37.4 kDa and the accession number is G8F337. |
种属 | Cynomolgus |
表达系统 | HEK293 Cells |
标签 | C-His |
蛋白编号 | G8F337 |
别名 | interleukin 1 receptor, type I |
蛋白构建 | A DNA sequence encoding the cynomolgus IL1R1 (EHH61701.1) (Met1-Thr332) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Asp 21 |
蛋白纯度 | > 95 % as determined by SDS-PAGE |
分子量 | 37.4 kDa (predicted); 47-57 kDa (reducing conditions) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Interleukin 1 receptor, type I (IL-1R1) also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. IL-1R1/CD121a is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA). IL-1R1/CD121a is an important mediator involved in many cytokines induced immune and inflammatory responses. This protein has been characterized by pharmacological and molecular techniques in the mouse brain. The spindle-shaped astrocytes enclose the wound, separating the healthy from damaged neural tissue. The shape change and subsequent repair processes are IL-1β activity-dependent, acting through the IL-1 type 1 receptor (IL-1R1), as co-application of the IL-1type 1 receptor antagonist protein (IL-1ra) blocks IL-1β induced effects. In the spleen, a slight increase in IL-1R ACP and IL-1R1 was observed during the first hours following LPS stimulation. In conclusion, IL-1R AcP mRNA is expressed in the brain and in other tissues where IL-1R1/CD121a transcripts are found. However, the regulation of its expression is distinct from IL-1R1/CD121a. The high level of expression and the lack of regulation of IL-1R AcP transcripts in the brain under inflammatory conditions suggest that the protein might be constitutively expressed in excess. |
版权所有©2015-2024 TargetMol Chemicals Inc.保留所有权利.