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The insulin family consists of insulin, insulin-like growth factor 1 (IGF-1), insulin-like growth factor 2 (IGF-2), their receptors (IR, IGF-1R and IGF-2R), and their binding proteins.Insulin-like growth factor I (IGF-I) is a polypeptide hormone produced mainly by the liver in response to the endocrine GH stimulus, but it is also secreted by multiple tissues for autocrine/paracrine purposes.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
100 μg | ¥ 1,470 | 5日内发货 | |
500 μg | ¥ 5,930 | 5日内发货 | |
1 mg | ¥ 9,890 | 5日内发货 |
生物活性 | 1. Immobilized Human IGF1R, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IGFI, hFc Tag with the EC50 of 0.62μg/ml determined by ELISA (QC Test).
2. Human IGFBP-3, His Tag captured on CM5 Chip via anti-his antibody can bind Human IGFI, hFc Tag with an affinity constant of 2.32 nM as determined in SPR assay. |
产品描述 | The insulin family consists of insulin, insulin-like growth factor 1 (IGF-1), insulin-like growth factor 2 (IGF-2), their receptors (IR, IGF-1R and IGF-2R), and their binding proteins.Insulin-like growth factor I (IGF-I) is a polypeptide hormone produced mainly by the liver in response to the endocrine GH stimulus, but it is also secreted by multiple tissues for autocrine/paracrine purposes. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | N-hFc |
蛋白编号 | P05019-1 |
别名 | 胰岛素样生长因子,MGF,IGF-I,IGF1A,IBP1 |
蛋白构建 | Gly49-Ala118 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 33.18 kDa (predicted). Due to glycosylation, the protein migrates to 38-43 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. |
研究背景 | The insulin family consists of insulin, insulin-like growth factor 1 (IGF-1), insulin-like growth factor 2 (IGF-2), their receptors (IR, IGF-1R and IGF-2R), and their binding proteins.Insulin-like growth factor I (IGF-I) is a polypeptide hormone produced mainly by the liver in response to the endocrine GH stimulus, but it is also secreted by multiple tissues for autocrine/paracrine purposes. |