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FCGRT & B2M Heterodimer Protein, Human, Recombinant (His & Avi), Biotinylated
产品编号 TMPY-05448
FCGRT & B2M Heterodimer Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 45.3 kDa and the accession number is NP_004098.1&NP_004039.1.
FCGRT & B2M Heterodimer Protein, Human, Recombinant (His & Avi), Biotinylated
产品编号 TMPY-05448
FCGRT & B2M Heterodimer Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 45.3 kDa and the accession number is NP_004098.1&NP_004039.1.
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 20 μg | ¥ 2,210 | 5日内发货 | |
| 100 μg | ¥ 7,790 | 5日内发货 |
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生物活性
| 生物活性 | 1. Measured by its ability to bind human IgG1 (Fc) in a functional ELISA.
2.Loaded Biotinylated Human FcRn recombinant protein (His & Avi Tag) on SA Biosensor, can bind IgG1 Fc (IgG1) with an affinity constant of 0.073 μM as determined in a BLI assay.
3.Loaded Biotinylated Human FcRn recombinant protein (His & Avi Tag) on SA Biosensor, can bind IgG4 Fc with an affinity constant of 0.070 μM as determined in a BLI assay.
4. The purity of Human FCGRT & B2M Heterodimer Protein (His & AVI Tag), Biotinylated is more than 95% and the molecular weight of this protein is around 46-56 kDa verified by SEC-MALS. |
| 产品描述 | FCGRT & B2M Heterodimer Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 45.3 kDa and the accession number is NP_004098.1&NP_004039.1. |
| 种属 | Human |
| 表达系统 | HEK293 Cells |
| 标签 | His, Avi |
| 蛋白编号 | NP_004098.1&NP_004039.1 |
| 别名 | Fc fragment of IgG, receptor, transporter, alpha,alpha-chain,Fc fragment of IgG, receptor, transporter, α,FCRN,α-chain |
| 蛋白构建 | A DNA sequence encoding the extracellular domain (Met1-Ser297) of human FCGRT (NP_004098.1) was fused with a C-terminal polyhistidine tagged AVI tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human B2M (NP_004039.1) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the human FCGRT/B2M heterodimer was purified. The purified heterodimer was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Ala 24 & Ile 21 |
| 蛋白纯度 | > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC |
| 分子量 | 45.3 kDa (predicted) |
| 内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
| 缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| 复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. |
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