FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated

产品编号 TMPY-05650

FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 45.3 kDa and the accession number is Q8SPV9&Q8SPW0.

FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated

产品编号 TMPY-05650

规格	价格	库存
20 μg	¥ 2,930	现货
100 μg	¥ 6,830	5日内发货
200 μg	¥ 11,650	5日内发货
500 μg	¥ 23,540	5日内发货

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生物活性

生物活性	1. Immobilized Anti-CD20 Antibody at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus FCGRT&B2M Heterodimer Protein (His & AVI Tag), Biotinylated, the EC50 is 80-240 ng/mL. _x000D_ 2. Loaded Biotinylated Cynomolgus FcRn recombinant protein ( His & Avi Tag ) on SA Biosensor, can bind IgG1 Fc with an affinity constant of 0.08 μM as determined in a BLI assay. _x000D_ 3. The purity of Cynomolgus FCGRT & B2M Heterodimer Protein (His & AVI Tag), Biotinylated is more than 90% and the molecular weight of this protein is around 46-56 kDa verified by SEC-MALS.
产品描述	FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 45.3 kDa and the accession number is Q8SPV9&Q8SPW0.
种属	Cynomolgus
表达系统	HEK293 Cells
标签	His, Avi
蛋白编号	Q8SPV9&Q8SPW0
别名	Fc fragment of IgG, receptor, transporter, alpha,Fc fragment of IgG, receptor, transporter, α
蛋白构建	The cynomolgus FCGRT (Q8SPV9) (Met 1-Ser 297) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus, constructed the plasmid 1; The cynomolgus B2M (Q8SPW0) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT/B2M heterodimer was purified. The purified heterodimer was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
蛋白纯度	> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
分子量	45.3 kDa (predicted)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.

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