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Ephrin A2/EFNA2 Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 19.4 kDa and the accession number is F1MA19.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
200 μg | ¥ 4,420 | 5日内发货 |
生物活性 | Measured by its ability to bind Mouse EphA4 hFc in functional ELISA. |
产品描述 | Ephrin A2/EFNA2 Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 19.4 kDa and the accession number is F1MA19. |
种属 | Mouse |
表达系统 | HEK293 Cells |
标签 | Tag Free |
蛋白编号 | F1MA19 |
别名 | Epl6,Elf1,Eplg6,Lerk6,CEK7L,ephrin-A2 |
蛋白构建 | A DNA sequence encoding the mouse EFNA2 (NP_031935.3) without the pro peptide (Met 1-Asn 184) was expressed and purified, with additional seven amino acid (ALEVLFQ) at the C-terminus. Predicted N terminal: Glu 23 |
蛋白纯度 | > 97 % as determined by SDS-PAGE |
分子量 | 19.4 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Ephrin-A2 also known as EFNA2 or EPH-related receptor tyrosine kinase ligand 6, is a member of the ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin-A2 and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. Ephrin-A2 regulates the position-specific affinity of limb mesenchyme and is involved in cartilage pattern formation in the limb. |
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