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EPhA10 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 58.8 kDa and the accession number is Q5JZY3-1.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
100 μg | ¥ 3,100 | 5日内发货 | |
500 μg | ¥ 12,400 | 5日内发货 | |
1 mg | ¥ 20,700 | 5日内发货 |
生物活性 | Immobilized Human EPHA10, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human/Cynomolgus EFNA3, hFc Tag with the EC50 of 0.24μg/ml determined by ELISA. |
产品描述 | EPhA10 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 58.8 kDa and the accession number is Q5JZY3-1. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-His |
蛋白编号 | Q5JZY3-1 |
别名 | MGC43817,FLJ33655,FLJ16103,EphA10 |
蛋白构建 | Glu34-Ala565 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 58.8 kDa (predicted). Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. |
研究背景 | EphA10 (erythropoietin-producing hepatocellular carcinoma receptor A10) is a catalytically defective receptor protein tyrosine kinase in the ephrin receptor family.EphA10 was elevated and higher in tumor tissues than in normal tissues in some cancer types, including pancreatic cancer.EphA10 silencing reduced the proliferation, migration, and adhesion of MIA PaCa-2 and AsPC-1 pancreatic cancer cells. EphA10 plays a pivotal role in the tumorigenesis of pancreatic epithelial cells and is a novel therapeutic target for pancreatic cancer. |
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