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EIF5 Protein, Human, Recombinant (GST)

产品编号 TMPY-03184

别名: EIF-5A, eukaryotic translation initiation factor 5, EIF-5

EIF5 Protein, Human, Recombinant (GST) is expressed in E. coli expression system with GST tag. The predicted molecular weight is 44.1 kDa and the accession number is P55010.

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EIF5 Protein, Human, Recombinant (GST)

规格	价格/CNY		货期	数量
100 μg	￥ 3,820		5日内发货

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生物活性	Testing in progress
产品描述	EIF5 Protein, Human, Recombinant (GST) is expressed in E. coli expression system with GST tag. The predicted molecular weight is 44.1 kDa and the accession number is P55010.

种属	Human
表达系统	E. coli
标签	N-GST
蛋白编号	P55010
别名	EIF-5A, eukaryotic translation initiation factor 5, EIF-5
蛋白构建	The mature form of Human EIF5 (P55010) (Met1-Asp150) was fused with the GST tag at the N-terminus.
蛋白纯度	> 95 % as determined by SDS-PAGE
分子量	44.1 kDa (predicted)

内毒素	Please contact us for more information.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	EIF-5A, also known as EIF5, functions in start site selection as a GTPase accelerating protein (GAP) for the eukaryotic translation initiation factor (eIF) 2•GTP•tRNA ternary complex within the ribosome-bound pre-initiation complex. In protein synthesis initiation, eIF2 functions in its GTP-bound state to deliver initiator methionyl-tRNA to the small ribosomal subunit and is necessary for protein synthesis in all cells. EIF-5A stabilizes the binding of GDP to eIF2 and is therefore a bi-functional protein that acts as a GDP dissociation inhibitor (GDI). EIF-5A also interacts with eIF1 and eIF3 and binds the eIF2-GTP/Met-tRNA ternary complex along with the 40S ribosome subunit.

参考文献

1. Si K,et al. (1996) Charactatierizon of multiple mRNAs that encode mammalian translation initiation factor 5 (eIF-5). J Biol Chem. 71(28):16934. 2. Das S,et al. (1998) Specific interaction of eukaryotic translation initiation factor 5 (eIF5) with the beta-subunit of eIF2. J Biol Chem. 272(50):31712-8. 3. Conte MR,et al. (2006) Structure of the eukaryotic initiation factor (eIF) 5 reveals a fold common to several translation factors. Biochemistry. 45(14):4550-8.

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Keywords

EIF5 Protein, Human, Recombinant (GST) EIF-5A EIF 5 eukaryotic translation initiation factor 5 EIF5 EIF-5 recombinant recombinant-proteins proteins protein

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