EGFR Protein, Human, Recombinant (His), AF488-Labeled

产品编号 TMPY-06990

EGFR Protein, Human, Recombinant (His), AF488-Labeled is expressed in HEK293 Cells. The accession number is P00533.

EGFR Protein, Human, Recombinant (His), AF488-Labeled

产品编号 TMPY-06990

EGFR Protein, Human, Recombinant (His), AF488-Labeled is expressed in HEK293 Cells. The accession number is P00533.

规格	价格	库存	数量
25 μg	¥ 3,430	期货
100 μg	¥ 10,900	期货

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产品信息

生物活性	1. Immobilized EGF Protein, Human, Recombinant (hFc) (Cat#TMPY-01062) at 2 μg/mL (100 μl/well) can bind EGFR Protein, Human, Recombinant (His), AF488-Labeled (Cat#TMPY-06990), the EC50 is 100-300 ng/mL. 2. Flow cytometric analysis of anti-EGFR CAR expression. 293 cells were lentivirally transduced with anti-EGFR CAR. Flow cytometric analysis was performed with a negative control protein and EGFR Protein, Human, Recombinant (His), AF488-Labeled (Cat#TMPY-06990), respectively. Non-transduced 293 cells were used as a control (left). 3. Binding activity of AF 488-conjugated EGFR protein to PBMC cells. PBMC cells were stained with anti-CD3 antibody and EGFR Protein, Human, Recombinant (His), AF488-Labeled (Cat#TMPY-06990) and detected by flow cytometry. PBMC cells stained with anti-CD3 antibody were used as a control (left).
产品描述	EGFR Protein, Human, Recombinant (His), AF488-Labeled is expressed in HEK293 Cells. The accession number is P00533.
种属	Human
表达系统	HEK293 Cells
标签	C-His
蛋白编号	P00533
别名	PIG61,NISBD2,mENA,HER1,ERRP,ERBB1,ERBB
蛋白构建	A DNA sequence encoding the Human EGFR (NP_005219.2) (Met1-Ser645) was expressed with a polyhistidine tag at the C-terminus. The protein is site-specifically labeled with AF 488 (Excitation Max. = 495 nm, Emission Max. = 519 nm).
蛋白纯度	≥ 90% as determined by SDS-PAGE.
分子量	72 kDa (predicted)
内毒素	< 1.0 EU per μg of the protein as determined by the LAL method
缓冲液	This product is Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
复溶方法	Please refer to the lot-specific COA.
存储	Twelve months from date of receipt at -20℃ to -70℃ in lyophilized form and 3 months at -70℃ under sterile conditions after reconstitution. Protect from prolonged exposure to light and avoid repeated freeze-thaw cycles.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

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