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Creatine kinase M/CKM Protein, Human, Recombinant is expressed in E. coli.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
250 μg | 待询 | 期货 |
生物活性 | Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
产品描述 | Creatine kinase M/CKM Protein, Human, Recombinant is expressed in E. coli. |
种属 | Human |
表达系统 | E. coli |
别名 | M-CK,CPK-M,CKMM |
蛋白纯度 | ≥ 90% as determined by SDS-PAGE. |
分子量 | 44.06 kDa (predicted) |
内毒素 | Please contact us for more information. |
缓冲液 | Lyophilized from sterile 20 mM Tris, 50mM NaCl, 1mM EDTA, 5mM β-ME, 50% Glycerol, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
复溶方法 | Please refer to the lot-specific COA. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. |
研究背景 | CKM, also known as CK-MM, is mainly expressed in skeletal muscle. As the primary CK isoenzyme, it also can be detected in heart muscle. CKM is a subunit of creatine kinase (CK). CK is an enzyme expressed by various tissues and cell types. It catalyses the conversion of creatine and consumes adenosine triphosphate (ATP) to create phosphocreatine and adenosine diphosphate (ADP). In the cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). |