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Creatine kinase B/CKB Protein, Human, Recombinant is expressed in E. coli.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
250 μg | 询价 | 期货 |
生物活性 | Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
产品描述 | Creatine kinase B/CKB Protein, Human, Recombinant is expressed in E. coli. |
种属 | Human |
表达系统 | E. coli |
别名 | HEL-S-29,HEL-211,CPK-B,CKBB,B-CK,BCK |
蛋白纯度 | ≥ 90% as determined by SDS-PAGE. |
分子量 | 43.6 kDa. (predicted) |
内毒素 | Please contact us for more information. |
缓冲液 | Supplied as sterile 20 mM Tris, 50mM NaCl, 1mM EDTA, 5mM β-ME, 50% Glycerol, pH 7.4. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
研究背景 | CKB(Creatine kinase B type) contains 1 phosphagen kinase C-terminal domain and 1 phosphagen kinase N-terminal domain. It belongs to the ATP:guanido phosphotransferase family. CKB consists of a homodimer of two identical brain-type CK-B subunits. CKB is a cytoplasmic enzyme involved in cellular energy homeostasis, with certain fractions of the enzyme being bound to cell membranes, ATPases, and a variety of ATP-requiring enzymes in the cell. There, CKB forms tightly coupled microcompartments for in situ regeneration of ATP that has been used up. CKB reversibly catalyzes the transfer of "energy-rich" phosphate between ATP and creatine or between phospho-creatine (PCr) and ADP. Its functional entity is a homodimer in brain, smooth muscle as well as in other tissues and cells such as neuronal cells, retina, kidney, bone etc. |