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CD16b Protein, Human, Recombinant (NA1, His & Avi), Biotinylated
产品编号 TMPY-06550
CD16b Protein, Human, Recombinant (NA1, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 23.75 kDa and the accession number is AAA35881.1.
CD16b Protein, Human, Recombinant (NA1, His & Avi), Biotinylated
产品编号 TMPY-06550
CD16b Protein, Human, Recombinant (NA1, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 23.75 kDa and the accession number is AAA35881.1.
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 20 μg | ¥ 3,820 | 5日内发货 | |
| 100 μg | ¥ 8,840 | 5日内发货 |
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生物活性
| 生物活性 | 1.Loaded Biotinylated Human FcγRIIIB / CD16b (NA1) recombinant protein (His & Avi Tag) on SA Biosensor, can bind Rituximab (IgG1) with an affinity constant of 4.3 μM as determined in BLI assay(QC tested).
2. The purity of CD16b Protein, Human, Recombinant (NA1 allotype, His & AVI Tag) is more than 90% and the molecular weight of this protein is around 26-44 kDa verified by SEC-MALS. |
| 产品描述 | CD16b Protein, Human, Recombinant (NA1, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 23.75 kDa and the accession number is AAA35881.1. |
| 种属 | Human |
| 表达系统 | HEK293 Cells |
| 标签 | His, Avi |
| 蛋白编号 | AAA35881.1 |
| 别名 | Fc fragment of IgG receptor IIIb,CD16,FCRIII,Fc gamma RIIIb,FCGR3,FCRIIIb,Fc γ RIIIb,FCR-10,FCG3,CD16b |
| 蛋白构建 | The Human FCGR3B (AAA35881.1) (Met1-Ser200) was expressed with a c-terminal AVI tagged polyhistidine tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. |
| 蛋白纯度 | > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC |
| 分子量 | 23.75 kDa (predicted) |
| 内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
| 缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| 复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. |
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