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CD16b Protein, Human, Recombinant (NA2,His & Avi), Biotinylated

产品编号 TMPY-05125
CD16b Protein, Human, Recombinant (NA2,His & Avi), Biotinylated is expressed in HEK293 mammalian cells. The predicted molecular weight is 24.1 kDa and the accession number is O75015.2.
规格价格库存数量
20 μg¥ 2,9405日内发货
100 μg¥ 6,8005日内发货
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产品信息

生物活性
1.Loaded Biotinylated Human FcγRIIIB / CD16b (NA2) recombinant protein (His & Avi Tag) on SA Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 3.6 μM as determined in a BLI assay.
产品描述
CD16b Protein, Human, Recombinant (NA2,His & Avi), Biotinylated is expressed in HEK293 mammalian cells. The predicted molecular weight is 24.1 kDa and the accession number is O75015.2.
种属
Human
表达系统
HEK293 Cells
标签C-Avi-His
蛋白编号O75015.2
别名
FCRIIIb,FCRIII,FCR-10,FCGR3,FCG3,Fc γ RIIIb,Fc gamma RIIIb,Fc fragment of IgG receptor IIIb,CD16b,CD16
蛋白构建
A DNA sequence encoding the human FCGR3B (O75015.2) (Met1-Ser200) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus (AVI-his). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Gly 17
蛋白纯度
> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC
分子量24.1 kDa (predicted); 40-50 kDa (reducing condition, due to glycosylation)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.

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