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Carbonic Anhydrase 10 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 27.2 kDa and the accession number is A0A384MTY8.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
20 μg | ¥ 2,270 | 5日内发货 |
生物活性 | Measured by its esterase activity. The specific activity is >100 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 1 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. |
产品描述 | Carbonic Anhydrase 10 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 27.2 kDa and the accession number is A0A384MTY8. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | Tag Free |
蛋白编号 | Q9NS85 |
别名 | HUCEP-15,CA-RPX,CARPX,carbonic anhydrase X,CA10 |
蛋白构建 | The mature form of human CARPX (NP_001076002.1) (Met 1-Asn 300) with five amino acids (DDDDK) at the C-terminus was expressed and purified. Predicted N terminal: Gln 22 |
蛋白纯度 | > 95 % as determined by SDS-PAGE |
分子量 | 27.2 kDa (predicted); 38 kDa (reducing condition, due to glycosylation) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 100 mM Glycine, 10 mM NaCl, 50 mM Tris, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Carbonic anhydrase X, also called carbonic anhydrase - related protein X (CARPX) and CA1, belongs to the CA family of zinc metalloenzymes which catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration, renal tubular acidification and bone resorption. The secreted protein CARPX without CA activity (hydration of CO2) is identified as an acatalytic member of the alpha-carbonic anhydrase subgroup. CARP X expression is detected in the adult total brain and almost all parts of the central nervous system, but not in the fetal brain. Accordingly, CARP X is suggested to play a role in the development of central nervous system, especially the brain. The same CARP X protein are encoded by multiple transcript variants of this gene. |
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