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Brk Protein, Human, Recombinant (GST) is expressed in Baculovirus insect cells with GST tag. The predicted molecular weight is 78 kDa and the accession number is Q13882-1.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μg | ¥ 3,170 | 5日内发货 |
生物活性 | Kinase activity untested |
产品描述 | Brk Protein, Human, Recombinant (GST) is expressed in Baculovirus insect cells with GST tag. The predicted molecular weight is 78 kDa and the accession number is Q13882-1. |
种属 | Human |
表达系统 | Baculovirus Insect Cells |
标签 | N-GST |
蛋白编号 | Q13882-1 |
别名 | protein tyrosine kinase 6,BRK |
蛋白构建 | A DNA sequence encoding the full length of human PTK6 (NP_005966.1) (Met 1-Thr 451) was fused with the GST tag at the N-terminus. Predicted N terminal: Met |
蛋白纯度 | > 89 % as determined by SDS-PAGE |
分子量 | 78 kDa (predicted); 70 kDa (reducing conditions) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Supplied as sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 10% glycerol. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 | It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. |
研究背景 | Tyrosine kinase (PTKs) is a protein that carries out tyrosine phosphorylation, which play a fundamental role in cell proliferation, survival, adhesion, and motility and have also been demonstrated to mediate malignant cell transformation. Overexpression of this protein in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Two classes of PTKs are present in cells: the transmembrane receptor PTKs and the non-receptor PTKs. Tyrosine kinase(PTKs)-6/ BRK is a cytoplasmic non-receptor protein kinase that may function as an intracellular signal transducer in epithelial tissues. Tyrosine kinase(PTKs)-6/ BRK has been shown to undergo autophosphorylation. It has been found that the constitutive expression of the tyrosine kinase(PTKs)-6/ BRK is in a large proportion of cutaneous T-cell lymphomas and other transformed T- and B-cell populations. State BRK expression was also induced in normal T-cells. In clinical, the cytoplasmic tyrosine kinase PTK6 (BRK) shows elevated expression in approximately two-thirds of primary breast tumours, and is implicated in EGF receptor-dependent signalling and epithelial tumorigenesis. |