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BDCA-2 Protein, Cynomolgus, Recombinant (hFc)

产品编号 TMPK-00504
BDCA-2 Protein, Cynomolgus, Recombinant (hFc) is expressed in HEK293 mammalian cells with N-hFc tag. The predicted molecular weight is 44.99 kDa and the accession number is A0A2K5UWP4.
规格价格库存数量
100 μg¥ 3,1005日内发货
500 μg¥ 12,4005日内发货
1 mg¥ 20,7005日内发货
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产品信息

生物活性
Immobilized Cynomolgus BDCA-2, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 13.1ng/ml determined by ELISA.
产品描述
BDCA-2 Protein, Cynomolgus, Recombinant (hFc) is expressed in HEK293 mammalian cells with N-hFc tag. The predicted molecular weight is 44.99 kDa and the accession number is A0A2K5UWP4.
种属
Cynomolgus
表达系统
HEK293 Cells
标签N-hFc
蛋白编号A0A2K5UWP4
别名
HECL,DLEC,CLECSF7,CLECSF11,CLEC4C,CD303,BDCA-2,BDCA2
蛋白构建
Tyr48-Ile212
蛋白纯度
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量44.99 kDa (predicted). Due to glycosylation, the protein migrates to 53-63 kDa based on Tris-Bis PAGE result.
内毒素< 1 EU/μg by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
BDCA-2, BDCA-3, and BDCA-4. In blood, BDCA-2 and BDCA-4 are expressed on CD11c(-) CD123(bright) plasmacytoid dendritic cells, whereas BDCA-3 is expressed on small population of CD11c() CD123(-) dendritic cells. All three Ags are not detectable on a third blood dendritic cell population, which is CD1c() CD11c(bright) CD123(dim), or on any other cells in blood.BDCA-2 is completely down-regulated on plasmacytoid CD11c(-) CD123(bright) dendritic cells, BDCA-2 is rapidly internalized at 37 degrees C after mAb labeling.

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