Mediates digestion of the cell walls of the 2 mating type gametes during mating as a necessary prelude to cell fusion. This enzyme acts specifically on the framework proteins (inner wall) of the cell wall, cleaving several model peptides at specific sites. Autolysin Protein, Chlamydomonas reinhardtii, Recombinant (His & SUMO) is expressed in E. coli expression system with N-6xHis-SUMO tag. The predicted molecular weight is 65.6 kDa and the accession number is P31178.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
20 μg | ¥ 2,290 | 20日内发货 | ||
100 μg | ¥ 4,320 | 20日内发货 | ||
1 mg | ¥ 14,600 | 20日内发货 |
产品描述 | Mediates digestion of the cell walls of the 2 mating type gametes during mating as a necessary prelude to cell fusion. This enzyme acts specifically on the framework proteins (inner wall) of the cell wall, cleaving several model peptides at specific sites. Autolysin Protein, Chlamydomonas reinhardtii, Recombinant (His & SUMO) is expressed in E. coli expression system with N-6xHis-SUMO tag. The predicted molecular weight is 65.6 kDa and the accession number is P31178. |
种属 | Chlamydomonas reinhardtii |
表达系统 | E. coli |
标签 | N-6xHis-SUMO |
蛋白编号 | P31178 |
别名 | Autolysin, GLE, Gametolysin, Gamete lytic enzyme |
氨基酸序列 | EIYAGKPIDLRTIVYIMDFSSCKLSGWSAPATLTPEKVTSDMLRGASAPTNNLANYYGACSYEKTLFNPDNFLVLGPVPVPCIGGVTPPPRPPRPPRPPPRAGSTISSLSRRNDTYDDWWDLSKYCTASEQQAWERAAEAYAQAIVAQDPNSATGKKLQGILQWRERRRNIYILPPGVKCSWSGYADVTCTSATCSAYVRGYSDTNAMQVIMHEAMHNYGLEHAGRGTLEYGDATDVMGDFNKAGKGLLCPNAPNMYRIGWAKPINEPGVAPFQNATGAWGNLTAANFTTDPWIRGLVIPAQGTRDDNMIVVNVGAQSTRDGAMKATGAQAYYFSYRIKNTTAGGYDSGLTLDFHKKVLVHAYNGIQSERVFGFKSNLLDWGPNFQSRSNTWTSPFLAYNNGLGGGVRLVVQSTSDTQAVVDICRISENGKELSCDDGIDNDCDGLQDNEDPDCQ |
蛋白构建 | 184-638 aa |
蛋白纯度 | > 90% as determined by SDS-PAGE. |
分子量 | 65.6 kDa (predicted) |
缓冲液 | Tris-based buffer, 50% glycerol |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
研究背景 | Mediates digestion of the cell walls of the 2 mating type gametes during mating as a necessary prelude to cell fusion. This enzyme acts specifically on the framework proteins (inner wall) of the cell wall, cleaving several model peptides at specific sites. |
bottom
您可能有的问题的答案可以在重组蛋白操作手册中找到
Autolysin Protein, Chlamydomonas reinhardtii, Recombinant (His & SUMO) Autolysin GLE Gametolysin Gamete lytic enzyme recombinant recombinant-proteins proteins protein