Tubulin polymerization-IN-37 is a potent inhibitor (IC50: 2.3 μΜ) of tubulin polymerization by binding specifically to the colchicine site of tubulin and preventing colchicine binding. It has potential applications in cancer research, notably for lymphomas [1].

Tubulin polymerization-IN-37 (compound 2f, 1 μΜ, 72 h) inhibits proliferation of lymphoma cells below 50% [1]. Tubulin polymerization-IN-37 (50 and 500 nM, 24-72 h) induces cell apoptosis and arrests cell in G2/M phase in VL51 and MINO cells [1]. Tubulin polymerization-IN-37 (5 μΜ) inhibits colchicine binding to tubulin by 80% [1]. Tubulin polymerization-IN-37 shows cytotoxicity against MCF-7 cells (IC 50 : 0.21 μΜ) [1]. Cell Proliferation Assay [1] Cell Line: VL51, MINO, HBL1, SU-DHL-10 cells. Concentration: 0-10 μΜ Incubation Time: 72 h Result: Inhibited cell proliferation to 28.1%, 8.6%, 7.3%, 13.8% at 1 μΜ, respectively. IC 50 s: 0.12, 0.07, 0.08, 0.07 μΜ, respectively. Cell Cycle Analysis [1] Cell Line: VL51 and MINO cells Concentration: 50 and 500 nM Incubation Time: 24, 48 and 72 h Result: Arrested cell in G2/M phase.