TOTO-3 (TOTO 3) serves as a versatile dye utilized extensively across various domains. This compound plays a crucial role in biological research by facilitating the observation and analysis of cell structures, tracking biomolecules, assessing cell functions, differentiating between cell types, and studying tissue pathology. Furthermore, TOTO-3 aids in the monitoring of microorganisms, significantly contributing to both foundational scientific research and clinical diagnostics. Beyond its scientific applications, the dye finds usage in traditional industries like textile dyeing and in innovative areas including functional textile processing, food coloring, and dye-sensitized solar cells.