TMX-4116, a casein kinase 1α (CK1α) degrader, preferentially degrades CK1α in MOLT4, Jurkat, and MM.1S cells, exhibiting degradation concentration 50s (DC50s) below 200 nM. TMX-4116 is utilized in the research of multiple myeloma [1].

TMX-4116 (compound 16; 1 μM; 4 h) shows a high degradation preference for CK1α in MOLT4 cells [1]. TMX-4116 (250 nM, 4 h) induces primary target degradation of CK1α, while no downregulation of PDE6D, IKZF1, and IKZF3 in MOLT4 cells [1]. Western Blot Analysis [1] Cell Line: MOLT4 cells Concentration: 1 μM Incubation Time: 4 hours Result: Showed a high degradation preference for CK1α. Western Blot Analysis [1] Cell Line: MOLT4, Jurkat, and MM.1S cells Concentration: 0 nM, 40 nM, 200 nM, 1 μM Incubation Time: 4 hours Result: Showed a high degradation preference for CK1α with the DC 50 value less than 200 nM in MOLT4, Jurkat, and MM.1S cells.