Steroid sulfatase/17β-HSD1-IN-1 is a highly effective inhibitor of both steroid sulfatase and 17β-hydroxysteroid dehydrogenase type 1 (17β-HSD1) activities, displaying an IC50 value of 28 nM against cellular human steroid sulfatase. This compound holds significant potential for investigating estrogen-dependent diseases [1].

Steroid sulfatase/17β-HSD1-IN-1 (compound 13) (20 μM; 48 h) has a low cytotoxicity in HEK-293 cells [1]. Steroid sulfatase/17β-HSD1-IN-1 (50-750 nM; 0-25 min) can inhibit T47D cells growth and 17β-HSD1 in a time-dependent manner [1]. Cell Cytotoxicity Assay [1] Cell Line: HEK-293 cells Concentration: 20 μM Incubation Time: 48 h Result: Exhibited a low cytotoxicity with the cell growth inhibition of 12.8%. Cell Proliferation Assay [1] Cell Line: T47D Concentration: 50, 100, 250, 500 and 750 nM Incubation Time: 0-25 min Result: Inhibited cell growth and 17β-HSD1 in a time-dependent manner.

Steroid sulfatase/17β-HSD1-IN-1 shows good metabolic stability in human hepatic S9 fraction (t 1/2 =47 min) and low intrinsic clearance Clint (15 μL/min/mg protein) [1].