Protriptyline is a potent antidepressant agent that inhibits AChE activity with an IC 50 value of 0.06 mM as well as inhibits Aβ Self-Assembly. Protriptyline has the potential to be used for depression and Alzheimer's disease [1] [2] [3].

Protriptyline (0-70 μM; 24 hours; PC3 cells) leads to cytotoxicity in PC3 cells [2]. Cell Cytotoxicity Assay [2] Cell Line: PC3 cells Concentration: 50, 60 and 70 μM Incubation Time: 24 hours Result: Concentration-dependently decreased cell viability.

Protriptyline (10 mg/kg; i.p.; for 21 days; rat model of AD) ameliorates spatial learning and retention memory in STZ treated rats [3]. Animal Model: Rat model of AD [3] Dosage: 10 mg/kg Administration: Intraperitoneal injection; for 21 days. Result: PRT treatment significantly reduced pTau, Aβ42 and BACE-1 levels, neurodegeneration, oxidative stress and glial activation which also significantly improved p-ERK/ERK ratio and enhanced BDNF and CREB levels by reducing NFκB and GFAP expression in STZ treated rats.