PRO-F, a photoactivable hydrogen sulfide (H2S) donor, possesses reactive oxygen species (ROS) scavenging properties and emits a fluorescent signal upon light activation, enabling real-time tracking of H2S release. Its activation is independent of endogenous substance consumption and facilitates intracellular H2S delivery, safeguarding cells from excessive ROS-induced damage. Notably, PRO-F has demonstrated efficacy in promoting chronic wound healing in diabetic models [1].

PRO-F (20 μM; 0-500秒) 可以依赖时间通过光激活 (365 nm) 来激活，实现激发波长λex=530 nm，发射波长λem=676 nm [1]。同样浓度下，PRO-F 在NDF和HaCaT细胞中于5分钟、10分钟及15分钟时间点产生红色荧光信号 [1]。