Pigment black 32 is a versatile dye used extensively in biological experiments to observe and analyze cell structures, track biomolecules, evaluate cell functions, differentiate cell types, detect biomolecules, investigate tissue pathology, and monitor microorganisms. Its applications extend from fundamental scientific research to a wide array of clinical diagnostics, and it is also prevalent in traditional domains like textile dyeing, as well as in innovative areas such as functional textile processing, food pigments, and dye-sensitized solar cells.