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Phyllanthusiin C 是一种从 Phyllanthus reticulatus 叶子红提取得到的天然化合物,具有抗菌活性,抑制脂多糖 (LPS) 诱导的 IC 细胞 RAW 264.7 产生一氧化氮,抑制微生物。
Phyllanthusiin C 是一种从 Phyllanthus reticulatus 叶子红提取得到的天然化合物,具有抗菌活性,抑制脂多糖 (LPS) 诱导的 IC 细胞 RAW 264.7 产生一氧化氮,抑制微生物。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
1 mg | ¥ 1,910 | 现货 | |
5 mg | ¥ 4,500 | 期货 | |
1 mL x 10 mM (in DMSO) | ¥ 7,800 | 期货 |
产品描述 | Phyllanthusiin C is a natural compound obtained from Phyllanthus reticulatus leaf red extract with antimicrobial activity and inhibits lipopolysaccharide (LPS)-induced nitric oxide production by IC cells RAW 264.7. |
体外活性 | This study was designed to obtain the chemical fingerprint and to investigate the effect of Phyllanthus urinaria on telomerase activity and apoptotic pathways in the human nasopharyngeal carcinoma cell line (NPC-BM1). METHODS AND RESULTS: The polyphenol compounds in P. urinaria were investigated by HPLC/MS. Cell viability with the treatment of P. urinaria, gallic acid, ellagic acid, quercetin and cisplatin was detected by MTT assay. TUNEL assay, DNA fragmentation analysis and caspase3 activity were used to confirm apoptotic changes. Telomerase activity was determined using the TRAP assay. RNA isolation and RT-PCR were used to analyze the related genes expression. All experiments on treatments with P. urinaria from 0-3 mg/ml were carried out for 24 h. 5 major compounds including gallic acid, brevifolin carboxylic acid, corilagin, Phyllanthusiin C and ellagic acid were identified as a plant fingerprint by HPLC/MS. With the MTT assay, we demonstrated that P. urinaria, gallic acid and ellagic acid reduce cell viability. The apoptosis features showed DNA fragmentation and increased caspase-3 activity associated with the down-regulation of Bcl-2, but not of Bax, p53, and PCNA (proliferating cell nuclear antigen) in P. urinaria-treated NPC-BM1 cells. Furthermore, treatment of NPC-BM1 cells led to an inhibition of hTERT (human telomerase reverse transcriptase), hTP1 (human telomerase-associated protein 1) and c-myc mRNA expression and to decreased telomerase activity. CONCLUSIONS: This study suggests that P. urinaria induces the death of NPC-BM1 cells in vitro through the induction of apoptosis and inhibited telomerase activity. |
别名 | 叶下珠鞣质 C |
分子量 | 926.65 |
分子式 | C40H30O26 |
CAS No. | 142674-52-0 |
Smiles | O=C1OCC2OC(OC(=O)C3=CC(O)=C(O)C(O)=C3)C4OC(=O)C5=CC(O)=C(O)C=6OC7(O)C(O)CC(O)(C(=O)OC2C4OC(=O)C8=CC(O)=C(O)C(O)=C8C=9C(O)=C(O)C(O)=CC19)C7C65 |
密度 | 2.22 g/cm3 (Predicted) |
存储 | store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
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