Olanexidine is a broad-spectrum antibacterial agent and antiseptic, effective against both Gram-positive and Gram-negative bacteria. It is commonly utilized in the research of infection and inflammation [1] [2] [3].

Olanexidine (50 μM, 48 h) exhibits broad-spectrum bactericidal activity against Gram-positive cocci and Gram-negative bacteria [1]. Olanexidine (50 μg/mL, 24 h) inhibits chronic inflammatory reactions in oral mucosal cells [2]. Olanexidine (Olanexidine gluconate, 0-15000 μg/mL) inhibits the binding ability of virus-like particles to the binding receptor of human norovirus and increases the aggregation of virus-like particles [3]. Cell Viability Assay [1] Cell Line: Gram-positive cocci, Enterococcus spp., Gram-positive bacilli, Gram-negative strains Concentration: 0-1 mg/mL approximately Incubation Time: 30 s Result: Inhibited bacterial activity with MIC values ranging from 6.8 to 1740 μg/mL approximately.

Olanexidine (1 -2% in saline, applied to the skin) is active against transient or resident bacterial flora on the skin of male cynomolgus monkeys [4]. Animal Model: Male cynomolgus monkeys [4] Dosage: 1 %, 1.5%, 2% in saline Administration: Applied to the skin Result: Showed the fast-acting and long-lasting bactericidal effects at 1.5% concentration.