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o-Pah 是一种含有 -NH2 和 -OH 取代的 meso-Ph 基团的 BODIPY 衍生物。在 Cu2+ 存在下,o-Pah 表现出金属诱导的 J 聚集效应,并对 Hg2+ 显示出特殊的荧光增强(Ex/Em=483/(495-600) nM)。
o-Pah 是一种含有 -NH2 和 -OH 取代的 meso-Ph 基团的 BODIPY 衍生物。在 Cu2+ 存在下,o-Pah 表现出金属诱导的 J 聚集效应,并对 Hg2+ 显示出特殊的荧光增强(Ex/Em=483/(495-600) nM)。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
10 mg | 询价 | 待询 | |
50 mg | 询价 | 待询 |
产品描述 | o-Pah, a BODIPY derivative with -NH2 and -OH substitutions on the meso-Ph group, demonstrates metal-induced J-aggregation when exposed to Cu2+ and shows specific fluorescence enhancement for Hg2+ (Ex/Em = 483/(495-600) nM) [1]. |
体外活性 | o-Pah (20 μM) changes color upon the addition of 100 μM various metal ions, with a further addition of 20 μM Cu 2+ in a DMSO/HEPES buffer solution [1]. Procedures for metal ion sensing: stock solutions of metal ions (100 mM and 10 mM) were prepared in deionized water [1]. A stock solution of o-Pah (0.5 mM) is prepared in DMSO and then diluted to 5 μM with HEPES buffer solution (50 mM KNO3, 50 mM HEPES, pH 7.2). During titration experiments, 2 mL solutions of o-Pah (5 μM, 0.5 μM) are placed in a 1 cm quartz optical cell, and Cu 2+, Hg 2+ stock solutions are added gradually with a micropipette. In selectivity experiments, test samples are prepared by adding appropriate amounts of the metal ion stock solution into the 2 mL solution of o-Pah (5 μM). During fluorescence measurements, the excitation wavelength is 483 nm, and emission spectra are collected between 495-600 nm. |
分子量 | 355.19 |
分子式 | C19H20BF2N3O |
CAS No. | 1181844-41-6 |
存储 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
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