N-[5-2-[Azo]phenyl]acetamide serves as a multifunctional dye used extensively across various fields. In the realm of biological experiments, this dye is instrumental for observing and analyzing cell structures, tracking biomolecules, evaluating cell functions, distinguishing cell types, detecting biomolecules, studying tissue pathology, and monitoring microorganisms. Its usage spans from fundamental scientific research to a broad spectrum of clinical diagnostics. Additionally, this compound finds applications in traditional industries like textile dyeing and emerging areas including functional textile processing, food pigments, and dye-sensitized solar cells.