Methyl-β-cyclodextrin (Methyl-beta-cyclodextrin) is a macrocyclic compound used as a solubilizer for hydrophobic compounds in biological experiments. Methyl-β-cyclodextrin is also a lipid raft inhibitor with cholesterol-lowering activity and potential antitumor activity.

PEL:3.33-4.23 mM.

方法：五种原发性渗出性淋巴瘤 (PEL) 细胞系 BCBL-1、BC-1、BC-3、TY-1 和 GTO 用 Methyl-β-cyclodextrin (0-10 mM) 处理 24 h，使用 MTT assay 检测细胞活力。
结果：Methyl-β-cyclodextrin 剂量依赖性抑制 PEL 细胞生长，IC50 在 3.33-4.23 mM 之间。[1]
方法：鸡肝癌细胞 LMH 用 Methyl-β-cyclodextrin (10 mM) 处理 1 h，然后在感染 FAdV-4 之前、期间和之后的不同时间点添加胆固醇 (50 µg/mL)，使用 Western Blot 检测靶点蛋白表达水平。
结果：当 LMH 细胞在感染前用增加浓度的 Methyl-β-cyclodextrin 预处理时，penton 蛋白水平以剂量依赖的方式降低。[2]

方法：为检测体内抗肿瘤活性，将 Methyl-β-cyclodextrin (500 mg/kg) 腹腔注射给携带 PEL 肿瘤 BCBL-1 的 NRJ 小鼠，每天一次，持续二十一天。
结果：Methyl-β-cyclodextrin 显著抑制 PEL 细胞的生长和侵袭，没有明显的不良反应。[1]
方法：为研究对胶原蛋白的调节作用，将 Methyl-β-cyclodextrin (1.25-5.0 mg/mouse) 皮内注射给 SKH1 小鼠，每周两次，持续两个月。
结果：Methyl-β-cyclodextrin 显示出强大的 COL I 上调活性，导致皮肤厚度增加。[3]

PEL cells are incubated in triplicate in a 96-well microculture plate in the presence of different concentrations of methyl-β-cyclodextrin (0-10 mM) in a final volume of 0.1 mL for 24 h at 37°C. Subsequently, MTT (0.5 mg/mL final concentration) is added to each well. After 3 h of additional incubation, 100 μL of a 0.04 N HCl is added to dissolve the crystals. Absorption values at 570 nm are determined.

PEL cells are incubated in triplicate in a 96-well microculture plate in the presence of different concentrations of methyl-β-cyclodextrin (0-10 mM) in a final volume of 0.1 mL for 24 h at 37°C. Subsequently, MTT (0.5 mg/mL final concentration) is added to each well. After 3 h of additional incubation, 100 μL of a 0.04 N HCl is added to dissolve the crystals. Absorption values at 570 nm are determined[1].