Mag-Fura-2 tetrapotassium

Mag-Fura-2 tetrapotassium 是紫外光激发的比率荧光指示剂 (Ex=334-360 nm, Em=510 nm)，用于检测 Mg2+ 和 Ca2+ 的浓度。

产品编号 T86850Cas号 132319-57-4

联系我们获取更多批次信息

资源下载

产品操作手册

Mag-Fura-2 tetrapotassium

产品编号 T86850Cas号 132319-57-4

Mag-Fura-2 tetrapotassium 是紫外光激发的比率荧光指示剂 (Ex=334-360 nm, Em=510 nm)，用于检测 Mg2+ 和 Ca2+ 的浓度。

TargetMol的所有产品仅用作科学研究或药证申报，不能被用于人体，我们不向个人提供产品和服务。请您遵守承诺用途，不得违反法律法规规定用于任何其他用途。

规格	价格	库存	数量
10 mg	询价	待询
50 mg	询价	待询

大包装 & 定制

加入购物车

实验操作小课堂

产品介绍

生物活性

产品描述

Mag-Fura-2 tetrapotassium, a UV-excitable fluorescent Mg²⁺/Ca²⁺ indicator (Ex=334-360 nm, Em=510 nm), is used to determine Mg²⁺ and Ca²⁺ concentrations [1] [2].

体外活性

Monitoring of Ca2+ release [1]: Incubate dispersed parotid acinar cells (e.g., acinar cells) with 8 μM Mag-fura-2 tetrapotassium for 30 min at 37°C. Wash cells twice with fresh HBSS-H without BSA. Precoat cell adhesive Cell-Tak on sample chambers. Transfer dye-loaded cells to the chambers and attach to the bottom. Mount the sample chambers on an inverted microscope (equipped with a 40x objective), and wash with BSA-free HBSS-H, followed by Mg2+/ATP-free ICM. Incubate acinar cells with Mg2+/ATP-free ICM containing 50 μg/mL saponin for 3-5 min at room temperature. Wash the cells with ICM containing Mg2+ and ATP, and incubate in the complete ICM for at least 5 min to allow complete filling of the intracellular Ca2+ stores. Alternately excite permeabilized cells, capture and digitize fluorescence emission at 510 nm using a digital imaging system, recording the 344 nm/360 nm ratio every 20 s. Note: ICM (intracellular-like medium) contains 125 mM KCl, 19 mM NaCl, 10 mM HEPES (pH 7.3 with KOH), 3 mM ATP, 1.4 mM MgCl2, 0.33 mM CaCl2, and 1 mM EGTA.

化学信息