Ibrutinib-biotin is a probe that consists of Ibrutinib linked to biotin via a long chain linker, has an IC50 of 0.755-1.02 nM for BTK.

BLK:3.66-4.27 nM (IC50), JAK3:5.34-4.87 μM (IC50), BMX:4.42-5.57 nM (IC50), ITK:0.187-0.198 μM (IC50), TEC:1.71-2.29 nM (IC50), BTK:0.755-1.02 nM (IC50), Lck:5.99-6.92 nM (IC50), Src:33.8-36.3 nM (IC50)

Ibrutinib-biotin (Compound 1-5) is a probe that consists of Ibrutinib linked to biotin via a long chain linker. Ibrutinib is a TEC family kinase inhibitor. The TEC kinase family is composed of five members, TEC, BTK (Bruton's Tyrosine Kinase), ITK (interleukin-2-inducible T-cell kinase)/EMT/TSK, BMX and TXK/RLK. The TEC family kinases participate in phosphotyrosine- mediated and phospholipid-mediated signaling systems. Many TEC family proteins are abundantly expressed in hematopoietic tissues, and play important roles in the growth and differentiation processes of blood cells[1].