HDAC8-IN-3 (compound P19) is a highly potent inhibitor of HDAC8, displaying an IC50 value of 9.3 μM and producing thermal stabilization. Moreover, HDAC8-IN-3 exhibits cytotoxicity and induces apoptosis specifically in leukemic cell lines [1].

HDAC8-IN-3 (compound P19) (5-200 μM, 48 hours; HEK293T cells) has cytotoxicity in leukemic cell lines [1]. HDAC8-IN-3 (compound P19) (50 μM) inhibits glucose transporter 1 (GLUT1)-mediated glucose transport by down-regulating GLUT1 expression (IC 50 = 28.2 μM) [1]. HDAC8-IN-3 (compound P19) (79.9 μM; 24 hours) can induce apoptotic death in the CEM cell line [1]. Cell Cytotoxicity Assay [1] Cell Line: K562, KCL22 and CEM leukemia cells. Concentration: 50 μM Incubation Time: 48 hours Result: Inhibited with IC 50 values of 79.9, 85.4 and 43.2 μM for CEM, K562 and KCL22 cells, respectively. Apoptosis Analysis [1] Cell Line: CEM cells Concentration: 79.9 μM Incubation Time: 24 hours Result: The percentage of apoptotic cells was recorded as 60.97%.