FtsZ-IN-1 is a potent FtsZ inhibitor with a quinoline ring and has a strong inhibitory effect on Gram-positive bacteria (MIC: 0.5-8 μg/mL).FtsZ-IN-1 enhances FtsZ polymerization and significantly promotes cell elongation of Bacillus subtilis.FtsZ-IN-1 has low hemolytic toxicity and low FtsZ-IN-1 exhibited anti-drug-resistant bacterial effects due to its low hemolytic toxicity and low tendency to induce drug resistance.