Ficain, an enzyme extract comprising multiple proteases, is derived from Ficus hispida L. and the latex of fig (Ficus carica). Its specificities vary in proportion with the ripening of the fruit. The compound finds extensive application in protein hydrolysis, food industries, and the production of bioactive peptides and antibody fragments [1] [2].

Ficain (0-8 µg/mL) 以酪蛋白作为底物，通过在280 nm波长处测定三氯乙酸滤液的吸光度来检测其酶活性 [2]。